Dhx9 atpase assay
WebJun 6, 2024 · The RNA helicase DHX9 associates with IGS-rRNA pRNA is a 250–300 nucleotide transcript that derives from processing of the 2-kb … WebOct 19, 2015 · The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from …
Dhx9 atpase assay
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WebAug 1, 2012 · The goal of this project is to develop HTS assays that will allow discovery of small molecule inhibitors of DHX9, which in turn, can be used in cell and animal models to examine the consequences of inhibition of this protein on cancer cell survival. ... Pelletier, Jerry (2015) Establishment of a Primary Screening Assay for the DHX9 Helicase ... WebSep 17, 2013 · Our ChIP assays indicate that DHX9 was stably bound to the H-DNA-forming region of the c-MYC promoter sequence. Thus, it is likely that the ChIP assays captured specific interactions between DHX9 and H-DNA in chromatin-plasmid complexes in human cells. ... FANCJ uses its motor ATPase to destabilize protein-DNA complexes, …
WebRNA-seq analysis showed that the Dhx9 deficiency caused an upregulated DNA damage response pathway and Gadd45, Cdkn1a, Cdc25, Wee1, and Myt1 expression to induce …
WebThe RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expr WebNational Center for Biotechnology Information
WebJul 30, 2024 · A co-IP assay was performed to assess the interaction of Flag-tagged WT and methylarginine binding-deficient (E691K) TDRD3 with endogenous DHX9 in MCF7 cells. The input and αFlag antibody-immunoprecipitated samples were analyzed by western blot using indicated antibodies. (D) DHX9 interacts with TDRD3 in an arginine methylation …
WebJ Immunol. Author manuscript; available in PMC 2013 May 17. Published in final edited form as: J Immunol. 2011 Nov 1; 187(9): 4501–4508. china post searchWebMay 8, 2024 · A top candidate DHX9 helicase promotes R-loop suppression and transcriptional termination. DHX9 interacts with PARP1, and both proteins prevent R … china post services trackingWebMar 5, 2024 · DExD/H-box RNA helicases (DDX/DHX) are encoded by a large paralogous gene family; in a subset of these human helicase genes, pathogenic variation causes neurodevelopmental disorder (NDD) traits and cancer. DHX9 encodes a BRCA1-interacting nuclear helicase regulating transcription, R-loops, and homologous recombination and … china post says deliveredWebOct 17, 2006 · Interacts (via DRBM, OB-fold and RGG regions) with NUP98 (via N-terminus); this interaction occurs in a RNA-dependent manner and stimulates DHX9-mediated ATPase activity and regulates transcription and splicing of a subset of genes (PubMed:28221134). grammaire interactiveWebMar 21, 2024 · Entrez Gene Summary for DHX9 Gene. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that … grammaire powerpointWebFeb 21, 2024 · We have shown using a combination of in vitro and in vivo assays that Nup98 directly binds DHX9 in the nucleoplasm and this interaction is facilitated by RNA. Importantly, binding of Nup98 to … china post small packageWebCCK-8 assay showed that DHX9 knockdown significantly inhibited the proliferation of MHCC97H cells at 48, 72, and 96 h (Figure 4B). In the scratch assay, DHX9 siRNA-treated cells covered almost 73% of scratches in 24 h and at least 85% in 48 h, while the cells in the NC group covered only 18% in 24 h and 25% in 48 h (Figure 4C,D). china post shanghai