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Transformation Protocol for Using NEB Golden - iGEM
WebGolden Gate assembly is useful for seamless assembly of multiple DNA fragments. It takes advantage of the separate recognition and cut sites of Type IIS restriction enzymes … WebApr 8, 2024 · Download Citation The type IIS restriction enzyme MmeI can cut across a double-strand break Background Type-IIS restriction enzymes cut outside their recognition sites, allowing them to remove ... filipino jobs in thailand
Golden Gate Cloning - Wikipedia
WebOverview of protocols. Basic Protocol 1 (BP1) describes the Golden Gate (GG) cloning reaction and is part of all other protocols. Basic Protocol 2 describes the domestication of a cloning vector to make a Golden Gate … WebThe NEBridge Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple … Web1 day ago · enzyme BsaI, which they called Golden Gate assembly7. Since then, the method’s popularity has grown8–10, due in part to its ability to connect fragments in a specific order and without a restriction scar11. Although the assembly of synthetic DNA by Golden Gate has clear utility in building large filipino jeepney font